Ethidium bromide laboratory safety environmental health and. Ethidium bromide 95% hplc homidium bromide sigmaaldrich. This eliminates the hazards of working with the powdered form. Are other fluorescent tags used instead of ethidium. Ethidium bromide etbr safety guidance document number. Ethidiun bromide is being used for nucleic acid staining. Ethidium bromide solution material safety data sheet sds. The detection limit of dna bound to ethidium bromide is 0. Ethidium bromide is a potent mutagen, often used in laboratories to stain nucleic acids in electrophoresis gels. Tufts university standard operating procedure sop for. Ethidium has several experimentally useful properties including binding to nucleic acids.
Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590. Dispose of the green bag in the biohazardous waste box for incineration. Ethidium bromide ethidium bromide2,7diamino10ethyl9phenylphenanthridinium bromide etbr remains a widely used dye for the visualization of nucleic acids in agarose gels. Bp210 synonyms no information available recommended use laboratory chemicals. Ethidium bromid e summary of data for chenflcal selection. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the usa. Ethidium bromide is strongly mutagenic, causing living cell mutations. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet light, providing molecular biologists with an excellent stain for visualizing and imaging nucleic acids. In the case of dna this is usually doublestranded dna from pcrs, restriction digests, etc. What links here related changes upload file special pages permanent link page information wikidata item cite this page. H504 application of the substance the mixturelaboratory chemicals details of the supplier of the safety data sheet manufacturersupplier.
No warranty, express or implied, is made and labchem inc. Ethidium bromide handling and disposal practices environmental. Well, we finally standardized gelred dye and we want to decontaminate all the working area. Ethidium bromide is commonly used to detect nucleic acids in molecular biology laboratories. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. Eye protection personnel handling ethidium bromide must wear adequate eye. Ethidium bromide solution sc286960 hazard alert code key. The results, as shown in table 1 and figure 3, indicate an optimal ethidium bromide concentration of 1. Etbr is also an irritant to the skin, eyes, mouth and upper respiratory tract.
Ethidium bromide is a potent mutagen used as a nucleic acid stain. Ethidium bromide laboratory spill and waste handling. Good work practices can help reduce hazardous exposure. It is mutagen or carcinogen, so be careful during the use better use gloves.
A safer alternative is to purchase ethidium bromide in solution. Ethidium bromide ethidium bromide ethidium bromide etbr is an intercalating agent commonly used as a nucleic acid stain in molecular biology laboratories for techniques such as agarose gel. Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards, precautions should be taken during handling and disposal. Ethidium bromide safe handling and disposal procedures. Ethidium bromide is typically purchased in powder or solution form and is soluble in water. Ethidium bromide solution catalog or product number. Product name ethidium bromide, 1% solutionmolecular biology cat no. Disposal, decontamination, and destruction procedure. Page 18 material safety data sheet university of lausanne. How to decontaminate laboratory equipment contaminated. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field. How to decontaminate the working zone with ethidium bromide hello dear friends. Eb was reported by watkins 1952 to be prepared from 3.
Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye. The toxic effects of etbr may be experienced if swallowed, inhaled, or absorbed through the skin. Ethidium bromide is typically in powder or solution form and is soluble in water. Ethidium bromide 2,7diamino10ethyl9phenylphenanthridinium bromide is used as a nucleic acid stain which fluoresces in the presence of ultraviolet uv light. Promega corporation 2800 woods hollow road madison, wi 53711 u. It fluorescents a redorange color under ultraviolet uv light and with increased. Ethidium bromide laboratory spill and waste handling procedures. Ethidium bromide is a toxic chemical and a mutagen. Ethidium bromide solutions must be treated as part of the experimental protocol or managed as a hazardous chemical waste and. Ethidium bromide etbr is commonly used as a nonradioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other methods of nucleic acid separation. It fluoresces a redorange colour under ultraviolet light and with increased fluorescence when bound to doublestranded dna.
The powder form is considered an irritant to the upper respiratory track, eyes and skin. Ethidium bromide fact sheet environmental health and safety. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. Ethidium bromide etbr, a dark red, crystalline, nonvolatile powder that is moderately soluble in water, is widely used in research laboratories as a visualizing agent for nucleic acids. The fluorescence of ethidium bromide in aqueous solution is significantly lower than that of the interchelated dye. Ethidium bromide ethidium bromide ethidium bromide etbr is an intercalating agent commonly used as a nucleic acid stain in molecular biology laboratories for techniques such as agarose gel electrophoresis. Place the green bag into the ethidium bromide solution. No producers of eb were reported in epas 1983 tscapp database cis, 1994. Material should be handled according to the material safety data sheet msds. Ethidium bromide is an intercalating agent commonly used as a fluorescent tag nucleic acid stain in molecular biology laboratories for techniques such as agarose gel electrophoresis. Dec 19, 20 ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. Protocol for rna quantitation using ethidium bromide etbr. It fluorescents a redorange color under ultraviolet uv light and with increased fluorescence when bound to doublestranded dna. Ethidium bromide is used to visualize dna in agarose gels and in many cesium chloride gradient protocols.
Bring information safety data sheet about ethidium bromide to medical center with you. Due to its unique structure, it can easily intercalate into dna strand. Ethidium bromide ethidium bromide2,7diamino10ethyl9phenylphenanthridinium bromide etbr remains a widely. Feb 27, 2020 ethidium bromide is a potent mutagen which has been commonly used as a nucleic acid stain for many years. Ethidium bromide is a dna intercalator, inserting itself between the base pairs in the double helix. Are other fluorescent tags used instead of ethidium bromide. Ethidium bromide etbr is commonly used as a nonradioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other. Wear gloves and a face mask when working with ethidium bromide in powdered form. It is commonly sold in a powder form which is soluble in water. Ultrapure ethidium bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and caesium chloride cscl gradients. Ethidium bromide ethidium bromide is an intercalating agent which resembles a dna base pair. Inspired by jason pattersons comment, i looked up ethidium bromide cattle and found a blog post about ethidium bromide toxicity. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and.
It is available as an aqueous solution containing 10 mgml. Etbr is a dark red, crystalline, nonvolatile solid, moderately soluble in water, which fluoresces. Apparently ethidium is routinely used to treat sleeping sickness in. Identification product name ethidium bromide, 1% solutionmolecular biology cat no. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and 25fold on binding doublestranded dna so that destaining the background is not necessary with a low stain concentration 10. How to decontaminate laboratory equipment contaminated with etbr. Its hazardous properties require safe handling and disposal procedures. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as. When exposed to ultraviolet light, it will fluoresce a redorange color, especially when it binds to dna 30fold increase. Wipe the area with a damp cloth after the work with ethidium bromide powder is complete. Review etbr material safety data sheet msds prior to handling the. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590 nm. Ethidium bromide safe handling, disposal, and emergency procedures. Charcoal filtration treatment is a simple and effective method for removing ethidium bromide from electrophoresis buffers.
Ethidium bromide etbr is commonly used as a nonradioactive dna stain to iden. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Material safety data sheet section 1 ethidium bromide 1 chemical product and company identification msds name. While not regulated as a hazardous waste, the mutagenic properties of this substance may present a. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes. How to decontaminate the working zone with ethidium bromide. Ethidium bromide solutions cannot be disposed of down the sanitary sewer. Ethidium bromide etbr is the most commonly used nucleic acid stain for page or agarose gel electrophoresis. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet.
The user must determine suitability of this information for his application. Ethidium bromide is an intercalating agent commonly used as a fluorescent tag nucleic acid. Filtering aqueous ethidium bromide waste solutions that are free of other contaminants through a bed of activated charcoal is a relatively simple and effective method for removal of ethidium bromide. The filtrate may be poured down the drain the used filters are disposed of similar to gels. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution. It is a phenanthridinium intercalator, structurally similar to propidium iodide. It is available as an aqueous solution containing 10 mgml ultrapure ethidium bromide and highpurity water. Sodium bromide 6 information in this msds is from available published sources and is believed to be accurate. Ethidium bromide, 10mgml aqueous solution safety data sheet according to federal register vol. The crystal or powder form is odorless and appears dark red in color. Mixture name product identifier % classification ghsus water, reagent grade, usp purified cas no 7732185 98 not classified. The mechanism of action for such compounds is known, in which these compounds are able to bind to the kinetoplastid dna and to alter their conformation to zdna molecules that stop replication of. Singlestranded rna can also be detected, since it usually folds back onto itself and thus provides local base pairing for the dye to intercalate.
Product ethidium bromide solution, 10ml product code h5041 substance number description amount symbols h504 ethidium bromide solution 1 t. Typically eb is purchased in powder or solution form and is soluble in water. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as provided for csc1 gradient protocols. Review the material safety data sheet msds prior to using this material. Safety data sheet creation date 08jun2010 revision date 18jan2018 revision number 4 1. Exposure information commercial availabili production and producers. Etbr can be absorbed through skin, so it is important to avoid any contact with the chemical. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910. A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry.
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